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The measurements were done in myocytes superfused with Tyrode's solution (1.2 mM Ca 2+ ) at 3536C and paced with field stimulation at 1 Hz as previously described (30, 31)

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Mechanistically, NNMT suppression increased adipose SAM and NAD, upregulated polyamine flux enzymes (ODC, SSAT), and altered histone methylation consistent with a shift toward oxidative metabolism [2]